How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Can anyone suggest why my PCR results gave big differences in the control group?

I did RT-PCR using SYBR Green and used 2 ul of cDNA per sample to investigate the effect of LPS in primary osteoclast culture. I loaded the same amount of RNA to make cDNA (550ng/sample). However...

17 September 2020 9,193 3 View

Does anyone have a recommendation for a book about Raman related to carbon besides Dresselhaus?

I need more than specifically graphene-related raman. I would like a book more related to changes in various carbon-chains. Thank you.

26 August 2020 5,101 3 View

Hi, can anyone clear up about the molarity concept?

I'm required to prepare 2mM of LiCl for my DNA extraction method, which I calculated to be 0.0212 g of LiCl in 250 ml of water. My supervisor told me to use any volume as long as it is 2 mM....

14 February 2020 9,808 3 View

I would like to find out why QGIS to Web is not used in academic research of Web GIS?

I would like to find out why QGIS to Web is not used in academic research of Web GIS?

15 May 2018 9,351 3 View

About online business acceptance journals?

Hi, Can someone please recommend what ISI or Scopus indexed journals that I can submit my manuscript ? my study is about what is people perception/acceptance of online business in asia? whether...

31 January 2018 7,181 7 View

How can we speed up the time of publishing students work, without turn a frustration for their career? Is it getting worse?

Some data suggest that wait times have increased within certain subsets of journals, such as popular open-access ones and some of the most sought-after titles. At Nature, the median review time...

10 February 2016 7,715 9 View

Where can i find Reves (1983) What makes a good language learner?

I'm looking for an often cited work in the field of Foreign language aptitude research, but which doesn't seem to be available (easily) online: Reves. T. (1983). What makes a good language...

01 January 1970 7,165 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View