What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

I would like to know if there is any report of the average volume of the lung lining fluid in BALB/c mice ?

I would like to know the volume of lining fluid in mice

24 April 2019 8,716 3 View

How can I measure encapsulation efficiency?

Hi, everyone! I have a drug with Platinum that is fat soluble, but my nanoliposomes are in aqueous suspension, so what is un-entrapped should be precipitate. I would like to measure encapsulation...

25 February 2019 7,623 1 View

Creating a new coordinate system in PETREL?

22 July 2018 7,099 14 View

HEK293 Cell line detachment after being adhered for over 3 days?

08 December 2016 9,523 2 View

Problems with the films for western blot developing?

Hi everyone! I am having some troubles when developing my western blot membranes using the film system: the films become really dark (even it is difficult to distinguish the bands) when I put them...

15 November 2016 891 7 View

Problems with the films for western blot developing?

Hi everyone! I am having some troubles when developing my western blot membranes using the film system: the films become really dark (even it is difficult to distinguish the bands) when I put them...

10 November 2016 7,734 8 View

What adherent substratum is appropriate for T cells, without any calcium current triggering?

24 October 2016 5,021 2 View

Best LC column for Plant Metabolomics?

Hi all!Typically we have been using a C18 Reverse Phase column for our studies, unfortunately there is no retention of most polar metabolites and all elute at very early retention times. This is,...

06 June 2016 8,133 10 View

How can I do a metabolite extraction for soil microorganisms?

Dear colleagues! I am trying to figure out how to do an extraction for soil microorganisms for further metabolomic analyses. I am only interested in the microbiota part, so I would like to discard...

08 December 2014 463 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View