Bovine serum albumin can detach A549 cells?

Hello all, I accidentally found that adding bovine serum albumin (BSA) in serum-free RPMI 1640 medium (1% w/v) caused A549 cell detachment. Have you known about that effect of BSA? I tried to find...

05 June 2016 9,659 2 View

What is the function of fetal bovine serum on degranulation of eosinophils, mast cells and neutrophils?

11 December 2015 9,577 3 View

Any advice on a MTT assay false positive result?

Hello all, I am using MTT assay to test the toxicity effect of a substance on A549 cells (adherent cell). I seed 5x10^4 cells/well of a 96 well-plate, incubate overnight for cell attachment. Then...

05 June 2015 6,982 10 View

How did you induce NET formation by IL-8 from neutrophils isolated from healthy donors?

Hello all, I am trying to induce NET induction by IL-8 (100 ng/ml) using healthy neutrophils and patients' neutrophils. I quantify the NET induction by Picogreen assay. In case of healthy...

04 May 2015 8,384 4 View

Does pressing hard when mounting cause cell morphology changes?

08 September 2014 9,158 2 View

Why do I get no plasmid after Maxiprep with MISSION shRNA plasmid DNA?

08 September 2014 3,793 11 View

What is the appropriate voltage for transferring LC3 in western blot?

I am using 80V/90min to transferring LC3 from 12% SDS-PAGE to PVDF membrane. But recently, I have not gotten any band of LC3. As I've read somewhere that LC3 could swim through the membrane if we...

04 May 2014 159 13 View

I got 2 bands for beta-actin in Western Blot when I used human neutrophils, but not for other cell lines. How can I solve this problem?

When I performed WB with cell lysate from human neutrophils, I got 2 bands of beta-actin. For other cell lines (HMC-1, LAD2 cells) I just only got 1 band for b-actin. I used the same antibody and...

03 April 2014 3,006 0 View

Does anyone have experience performing Western Blot with a low molecular weight protein?

I used to performed Western Blot to detect a protein with #14-16 kDa molecular weight (LC3) a few months ago. It worked very well. I have stopped doing that experiment for one month. I am now...

02 March 2014 6,743 10 View

Skimmilk as blocking buffer for ELISA?

Hi all, Can Skimmilk be used as blocking buffer for ELISA tests with human serum samples? Can anti-milk-IgG or IgE antibody in serum samples interacts with skimmilk that blocks the plate? Thanks...

01 February 2014 9,410 18 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View