Bovine serum albumin can detach A549 cells?

Hello all, I accidentally found that adding bovine serum albumin (BSA) in serum-free RPMI 1640 medium (1% w/v) caused A549 cell detachment. Have you known about that effect of BSA? I tried to find...

05 June 2016 9,659 2 View

What is the function of fetal bovine serum on degranulation of eosinophils, mast cells and neutrophils?

11 December 2015 9,577 3 View

Any advice on a MTT assay false positive result?

Hello all, I am using MTT assay to test the toxicity effect of a substance on A549 cells (adherent cell). I seed 5x10^4 cells/well of a 96 well-plate, incubate overnight for cell attachment. Then...

05 June 2015 6,982 10 View

How did you induce NET formation by IL-8 from neutrophils isolated from healthy donors?

Hello all, I am trying to induce NET induction by IL-8 (100 ng/ml) using healthy neutrophils and patients' neutrophils. I quantify the NET induction by Picogreen assay. In case of healthy...

04 May 2015 8,384 4 View

Does pressing hard when mounting cause cell morphology changes?

08 September 2014 9,158 2 View

Why do I get no plasmid after Maxiprep with MISSION shRNA plasmid DNA?

08 September 2014 3,793 11 View

Knockdown gene in LAD2 cells?

Hello all, I would like to knockdown some gene in LAD2 cells to produce a stable expressing cell line. Could you suggest me an appropriate method to do? Because LAD2 cells are stem cells and they...

05 June 2014 981 0 View

What is the appropriate voltage for transferring LC3 in western blot?

I am using 80V/90min to transferring LC3 from 12% SDS-PAGE to PVDF membrane. But recently, I have not gotten any band of LC3. As I've read somewhere that LC3 could swim through the membrane if we...

04 May 2014 159 13 View

Does anyone have experience performing Western Blot with a low molecular weight protein?

I used to performed Western Blot to detect a protein with #14-16 kDa molecular weight (LC3) a few months ago. It worked very well. I have stopped doing that experiment for one month. I am now...

02 March 2014 6,743 10 View

Skimmilk as blocking buffer for ELISA?

Hi all, Can Skimmilk be used as blocking buffer for ELISA tests with human serum samples? Can anti-milk-IgG or IgE antibody in serum samples interacts with skimmilk that blocks the plate? Thanks...

01 February 2014 9,410 18 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View