I want to check Cytokine (IL6 ,TNF-alpha ) levels in the Rabbit after wound creation with and without treatment . I am planning to do ELISA quantification. As per my research Blood plasma would be the best analyte . where from should I draw blood to get the best result . Or any other suggestion or reference ?
Hello Pratik Das
The best site for blood collection in rabbit depends on the volume and number of samples needed.
Generally, the marginal ear vein is the most common site for collection of small blood samples in the unanesthetized rabbit and can be used for single or repeat samples of 1–5 ml depending on the size and strain of the rabbit. This amount of blood sample should be sufficient enough for ELISA.
The rabbit is restrained and the ear is warmed in order to dilate the vessel. This may be done by gently stroking. The collection site should be shaved with an electric clipper and cleaned with three alternating scrubs of 70% alcohol and betadine. You may collect blood from the vein near the base of the ear, with successive samples (if necessary) taken from the alternate ear or by moving distally toward the tip of the ear. A 25G or 26G needle or butterfly catheter may be inserted into the vein while pressure applied to the vein at the base of the ear.
Best.
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