Kindly tell the exact recipe (concentration)for making 10 mM Tris base and 1mM Edta ?
1M EDTA is difficult, prepare 0.5M EDTA and adjust the pH to 8.0, otherwise, difficult to dissolved.
the 1M tris need to adjust the pH that you need.
Hi Maryam Wahab
briefly, You should weigh 1.211 g Tris (Molar mass: 121.14 g/mol) and 0,372 g EDTA ( Molar mass: 372.24), then add enough water to make it 1000ml.
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