I'll start working with kg1a cells. after the cell passage, the apoptotic occupations are not removed, gradually their number in the environment increases and they cause problems. How could you get rid of the apoptotic occupations?
Hello Zeinab Basiri
Are you referring to dead cells in culture?
If yes, then you may eliminate the dead cells from culture by collecting the cell suspension containing both the live and dead cells in the centrifuge tube, and centrifuge at low speed (100g for 10 mins). The live cells will form a pellet at the bottom of the centrifuge tube, and the dead cells and debris can be removed by discarding the supernatant. This is because the dead cells are fractured and therefore the dead cells and debris are lighter than live cells and will remain in the supernatant.
Best.
In which medium are you cultivating the cells? The recommendation is Iscove's Modified Dulbecco's Medium supplemented with 20% FBS.
If you are observing a buildup of apoptotic cells after cell passage in your KG1a cell line, there are several steps you can take to remove them and maintain a healthy culture:
All the best
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