I am trying to grow Kasumi-1 cell line. I use RPMI 1640 and 20 % FBS but cells never seem to be growing. They keep on shrinking in size and eventually die off.
Yes, L glutamine might help. I normally might put sodium pyruvate (for their ATP production), sodium bicarbonate (for best buffering condition since the cells is growing in CO2 incubator) and you might want to test the final medium to be near to PH7.4. Freshney book on tissue culture might help too. Good luck
i am facing the same problem now, i have added L-glutamine, sodium bicarbonate and HEPES . but still cells are not growing well. any other suggestions that might help?