10 Questions 22 Answers 0 Followers
Questions related from Rakeshkumar Yadav
Hello all, I have been trying to quantify a 292 bp in my community DNA. In order to quantify it, I am using absolute quantification approach. I amplified the gene (292bp), purified it (ratio...
28 May 2021 8,086 2 View
Hello, I was analysing my microbiome data for Beta diversity. In order to understand the significance of the location on the microbial community, I applied two test i.e. Permanova to assess...
24 March 2020 4,280 0 View
My goal is to align my metagenomic sequences with virulence gene database in order to find out which probable genes are prevalent in the data. So I ran diamond aligner using following...
28 November 2019 472 2 View
I am analysing a data that has normal distribution confirmed by Shapiro-Wilk Normality test (p=0.56) and Q-Q plot. I divided my data in to two groups. Following which, I applied Welsch test...
11 September 2019 2,834 7 View
I am analyzing a meta genomic data and I want to comment about the significance of beta diversity for the samples taken from different locations. In order to do that i did Adonis test (seed of...
07 August 2019 429 3 View
I am looking to validate the information generated from metagenomic sequencing. I know that I can carry out qPCR to validate my data. And I guess Random forest regression analysis can be done too....
23 April 2019 4,576 1 View
We all know about the specificity of viruses or phages and I guess that would specially limit their enrichment to certain groups. Suppose I have a sediment or sewage sample and I want to enrich...
26 September 2018 983 14 View
I am trying to understand mantel test in terms of getting to a biological conclusion. I referred few papers but failed to understand it completely. I have understood that it is a correlation...
21 September 2018 6,842 1 View
I have planned to use ZM,GPY, a minimal media, and one rich medium for Bacteria? kindly suggest an addition, if any. For cyanobacteria I am planning to use ASN III medium, kindly suggest an...
30 December 2017 1,028 4 View
source organism is also known. nearest match for structure with 80% similarity is found. is it possible?
01 December 2016 7,186 2 View