Rakeshkumar Yadav

13 Questions 29 Answers 0 Followers

Questions related from Rakeshkumar Yadav

Why my quantitative real-time standard curve efficiency never increases to 90 percent?

Hello all, I have been trying to quantify a 292 bp in my community DNA. In order to quantify it, I am using absolute quantification approach. I amplified the gene (292bp), purified it (ratio...

28 May 2021 8,239 2 View

How do I infer following results of Permanova and Beta disper test?

Hello, I was analysing my microbiome data for Beta diversity. In order to understand the significance of the location on the microbial community, I applied two test i.e. Permanova to assess...

24 March 2020 4,355 0 View

How do I decide the parameters to run diamond aligner?

My goal is to align my metagenomic sequences with virulence gene database in order to find out which probable genes are prevalent in the data. So I ran diamond aligner using following...

28 November 2019 528 2 View

Is it always necessary to apply FDR correction after Welsch's t-test?

I am analysing a data that has normal distribution confirmed by Shapiro-Wilk Normality test (p=0.56) and Q-Q plot. I divided my data in to two groups. Following which, I applied Welsch test...

11 September 2019 2,908 7 View

Do i need to do betadispersion after Adonis test?

I am analyzing a meta genomic data and I want to comment about the significance of beta diversity for the samples taken from different locations. In order to do that i did Adonis test (seed of...

07 August 2019 510 3 View

Please suggest any bioinformatic tools or lab-based experiments to validate the metagenomic data?

I am looking to validate the information generated from metagenomic sequencing. I know that I can carry out qPCR to validate my data. And I guess Random forest regression analysis can be done too....

23 April 2019 4,649 1 View

How does bile salt inhibits most of the Gram positive organism?

I was just wondering this when is was reading about media like Mackonkey. Just out of curiosity, I read few of the articles. Is it due the presence of teichoic acid in the CW of gram positive...

01 January 2019 9,440 1 View

Wondering how to enrich or concentrate phages or viruses for community phage/virus nucleic acid extraction and that too without using host?

We all know about the specificity of viruses or phages and I guess that would specially limit their enrichment to certain groups. Suppose I have a sediment or sewage sample and I want to enrich...

26 September 2018 1,061 14 View

Can any one explain Mantel Test in simple terms ?

I am trying to understand mantel test in terms of getting to a biological conclusion. I referred few papers but failed to understand it completely. I have understood that it is a correlation...

21 September 2018 6,906 1 View

Just out of curiosity. Why in AFLP there is a need of utilizing two types of restriction enzymes?

Why rare as wells as frequent cutters are used in AFLP? Was trying to think but couldn't reach to a conclusion !! Please comment !

27 February 2018 6,988 6 View

Please suggest the types or names of culture media (with ref paper) which i can use for maximum capturing of cultured diversity in marine Sponges?

I have planned to use ZM,GPY, a minimal media, and one rich medium for Bacteria? kindly suggest an addition, if any. For cyanobacteria I am planning to use ASN III medium, kindly suggest an...

30 December 2017 1,180 4 View

Relation between pairwise distance and intraspecies divergence?

how to interpret intraspecies divergence after calculating pairwise distance. are there any cutoff values to comment on high or low intraspecies divergence?

12 April 2017 720 2 View

How one can predict the metabolic pathway using bioinformatic tools( if any) for a novel compound, if the structure of compound is known ?

source organism is also known. nearest match for structure with 80% similarity is found. is it possible?

01 December 2016 7,264 2 View