12 Questions 27 Answers 0 Followers
Questions related from Rakeshkumar Yadav
Hello all, I have been trying to quantify a 292 bp in my community DNA. In order to quantify it, I am using absolute quantification approach. I amplified the gene (292bp), purified it (ratio...
28 May 2021 8,145 2 View
Hello, I was analysing my microbiome data for Beta diversity. In order to understand the significance of the location on the microbial community, I applied two test i.e. Permanova to assess...
24 March 2020 4,290 0 View
My goal is to align my metagenomic sequences with virulence gene database in order to find out which probable genes are prevalent in the data. So I ran diamond aligner using following...
28 November 2019 482 2 View
I am analysing a data that has normal distribution confirmed by Shapiro-Wilk Normality test (p=0.56) and Q-Q plot. I divided my data in to two groups. Following which, I applied Welsch test...
11 September 2019 2,840 7 View
I am analyzing a meta genomic data and I want to comment about the significance of beta diversity for the samples taken from different locations. In order to do that i did Adonis test (seed of...
07 August 2019 441 3 View
I am looking to validate the information generated from metagenomic sequencing. I know that I can carry out qPCR to validate my data. And I guess Random forest regression analysis can be done too....
23 April 2019 4,587 1 View
I was just wondering this when is was reading about media like Mackonkey. Just out of curiosity, I read few of the articles. Is it due the presence of teichoic acid in the CW of gram positive...
01 January 2019 9,346 1 View
We all know about the specificity of viruses or phages and I guess that would specially limit their enrichment to certain groups. Suppose I have a sediment or sewage sample and I want to enrich...
26 September 2018 990 14 View
I am trying to understand mantel test in terms of getting to a biological conclusion. I referred few papers but failed to understand it completely. I have understood that it is a correlation...
21 September 2018 6,852 1 View
I have planned to use ZM,GPY, a minimal media, and one rich medium for Bacteria? kindly suggest an addition, if any. For cyanobacteria I am planning to use ASN III medium, kindly suggest an...
30 December 2017 1,090 4 View
how to interpret intraspecies divergence after calculating pairwise distance. are there any cutoff values to comment on high or low intraspecies divergence?
12 April 2017 665 2 View
source organism is also known. nearest match for structure with 80% similarity is found. is it possible?
01 December 2016 7,194 2 View