The instrument is old, I changed a lot of the other plumbing. The pressure is fine and steady when equilibrating, then when it goes to make the injection, it just starts flowing from the needle and not through the brand new needle seat...
Thanks, it ended up being the rheodyne valve, it was sticking so I had to take it apart, clean it and replace the stator.
I am running several matrices for sugar on the HPLC, and no matter how clear the sample looks, the pressure jumps when it gets to this particularly starchy sample. I suspect that the starch is...
02 March 2014 7,083 34 View
I have gotten the Vitamin C to stick to a C18 using methanol at 0.5 mL/min, but it always comes out in a blob when I run a food sample. I have set the UV to 243, 250 and 260 to try and phase out...
31 December 2013 8,611 26 View
At times, I have run into analytes that don't give particularly great peak shape, whether on GC, HPLC or MS. I have run methods where despite some tailing on the chromatography, we have...
09 October 2013 5,204 3 View
I have obviously gathered validation data such as linearity tests, matrix spikes, precision and even blind proficiency samples. So what would be the next step?
08 September 2013 7,023 10 View
I cleaned the inlet, changed the liner and septum, the flows are good, cleaned the detector, changed the jet, recut both ends and reinstalled the column. I could see a solvent peak after that, but...
07 August 2013 5,856 17 View
My lab analyzes sugar in food samples using AOAC guidelines, and it doesn't take long for the chromatographic performance to decline, and there is a steady increase in backpressure with every run....
07 August 2013 6,717 5 View
The work will not be for any educational purposes, this will be for my business institution. We are on a tight budget, but I also don't want to skimp too much on quality.
06 July 2013 9,915 8 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
effect of double bond on the flexibility of polymers
07 April 2021 0 0 View
I am writing to request some information for making polymer color flakes. I would appreciate the help.
17 March 2021 0 0 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View