At times, I have run into analytes that don't give particularly great peak shape, whether on GC, HPLC or MS. I have run methods where despite some tailing on the chromatography, we have successfully passed blind proficiency samples time and again, but I fear if we were trying to get accredited for that method, the auditor may question the tailing. What are some thoughts on peak shape, and its importance to the method?
peak shape such as peak tailing and double peaks.
The majority of cases are caused by inappropriate conditions of separation which include improper selection of a column or solvent.
The simplest way is to examine the column performance according to "shipping inspection criteria" in the column inspection report attached to the column. If the examination reveals no peak shape anomalies, the cause will be an inappropriate separation condition. The separation condition such as eluent selection must be reconsidered.
If the same examination reveals any anomaly, the column can be deficient. Flushing (when the impurity could have accumulated) or replacement of the column is necessary. We recommend examining column performances on a regular basis and under the identical conditions.
read this for more information,
peak shape such as peak tailing and double peaks.
The majority of cases are caused by inappropriate conditions of separation which include improper selection of a column or solvent.
The simplest way is to examine the column performance according to "shipping inspection criteria" in the column inspection report attached to the column. If the examination reveals no peak shape anomalies, the cause will be an inappropriate separation condition. The separation condition such as eluent selection must be reconsidered.
If the same examination reveals any anomaly, the column can be deficient. Flushing (when the impurity could have accumulated) or replacement of the column is necessary. We recommend examining column performances on a regular basis and under the identical conditions.
read this for more information,
Peak shape is important for accuracy and precision of the results.
Merged peaks, trailing peak will not give you the very precise results.
It depends on method of analysis, constant condition of equipment GC, HPLC , temp , pressure, column, column pressure, column packing's, flow rate , mobile
phase or eluent selection, sample concentration, column selection/ choice , internal packing of the column etc...
Choose the Appropriate method of analysis...
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