I'm continuing my MPH thesis and I have to run a HPLC machine and analyze my milk sample. So I'm looking for training or any other resources which will be fulfill my demand.
The suppliers offer on site Training, but of course not for free. Search near to you for a group who is experienced in hplc and ask for help or an internship (it will Take same time). When you`ll have got Training, it is always helpful to Browse William Letter`s blog.
There are two types of information that you need:
- Theoretical training in chromatography. You should know some of that already if you are running TLC, such as the concepts of "strong" and "weak" solvents, mobile phase selectivity, and stationary phase selectivity. You can get this information from blogs or general reading. General knowledge for HPLC will include how to develop a robust analytical method, limits of detection (LoD), limits of quantitation (LoQ), detection techniques, and quantitation. There is more (use of blanks, references, etc) but you get the picture, I hope.
- Instrument specific training. This comes from the manufacturer, ideally. They may have videos on their web site or youtube.com. You may get some of it from your colleagues, or whoever maintains the system. This will tell you how to implement the method you use to measure the residues using the instrument controls and programming on the system, how to set the wavelengths chosen, and how to run other detectors (mass spectrometer or ELSD, for example). The instrument manufacturers sometimes have basic information as mentioned in the first point, but tending to be specific to their systems.
The first bullet is like learning the rules of the road and how to drive a car in various conditions which would be common to all cars. The other bullet is like learning where the gas tank cover release or heat controls are located within the car and vary in different makes and models of cars.
You need to work with a an HPLC instrument to obtain the hands on training. If you have an HPLC with you, probably you can ask for your supervisor. YouTube is also a good source but you do need to work with the instrument to learn it.
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