What are typical methods of producing HEA's?

I've been curious about how exactly HEA's are manufactured. Are they predominately cast? Printed with additive manufacturing?

29 December 2019 6,887 2 View

Is there a known method for the inhibition of aluminosilicate gel formation at high pH e.g. chelation of aluminium?

Looking for a method to create a solution containing dissolved aluminium and silicate but without the gel formation.

04 July 2019 3,683 1 View

Qiagen Blood & Tissue Kit with plants?

I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material? Thanks, Matt

24 March 2019 8,520 2 View

Are there theoretical limits to how many viable T cells can be derived from peripheral blood donation (e.g., PBMCs) of a single healthy donor?

Immune oncology drug programs are increasingly moving towards use of allogeneic T cells which can be engineered and used with unmatched recipients. Celyad, for instance, recently got clearance...

15 August 2018 1,122 2 View

Can isoelectric points of my protein affect nickel affinity chromatography?

When purifying my his-tagged protein much of it was eluting at only 50mM imidazole. The protein was binding to the charged beads as shown by samples from the beads and supernatant after incubation...

30 March 2018 5,096 4 View

Negative % degree of hydrolysis values for OPA assay?

Hi, I am using the o-phthalaldehyde assay (OPA) to quantify the % degree of hydrolysis in bacterial fermented milk supernatants. However, for some of my isolates I am obtaining negative %DH...

22 February 2018 2,459 3 View

How long does it generally take to recieve the full-text article/journal?

I have made several requests this afternoon for full-text articles/Journals. I am a new user to this site and am just curious how long it generally takes to process those requests. Thank you!

11 December 2017 4,893 5 View

Are there any recommendations on how to clean a biochemistry laboratory dishwasher?

Our biochemistry lab received a dishwasher from an agriculture lab. We were wondering if there are any recommendations on how to clean the dishwasher before we use it ourselves.I'm assuming we...

15 March 2017 3,072 1 View

Do all Pseudomonas aeruginosa fluoresce under UV light?

17 February 2017 9,895 2 View

What is the 'rescue' of an inhibitory effect (in order to rule out toxicity)?

I am going over a reviewer's comments for my paper and being new to the research world I am not sure what the reviewer means when he/she is asking me whether the inhibitory effect of my product...

10 September 2016 9,255 7 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View