I am trying to make an CV electrode using my GO-Electrolyte based gels mounted on glassy carbon. But after few minutes the mounted gel is coming out into the electrolyte solution (KOH). How can i solve this problem.?
I am exploring the effect of cross-linking between the graphene oxide nanosheets by mono and multivalent cations. How a metal ion reacts with GO nanosheets and what chemical changes it will...
08 September 2018 9,594 3 View
I am trying to calculate the lateral dimensions of prepared graphene oxide nano-sheets dispersed in water, several articles have indicated that UV Vis can be used as a tool to do so . However, i...
01 February 2018 8,193 3 View
I am studying the effect of extended ultrasonication on the synthesized graphene oxide nanosheets ( specially on the activity of surface oxy-functional groups ).
31 December 2017 9,070 6 View
I am preparing the GO nanosheets using Hummer's process, but synthesized nanosheets are of very high polydispersity in terms of lateral dimensions. Is there any method by which the polydispersity...
11 December 2017 411 3 View
I have synthesized Graphene oxide based gels through cross-linking of GO nanosheets by metal ions of various valencies. What should be the optimum degassing conditions for its lyophilized form ?
10 November 2017 4,497 5 View
I have an aqueous solution of exfoliated graphene oxide ( by ultrasonication) , Now if i wish to segregate these nanosheets on the basis of their lateral dimesions, how can i do so.?
09 October 2017 9,744 3 View
I am looking for the stability of aqueous dispersions of graphene oxide at various pH, can anyone describe me what should me my actual methodology to do so.? Also why these dispersions are most...
05 June 2015 4,299 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I have tried to saturate ferritic steel with hydrogen using the electrolytic method and H2SO4. However, the effect was very poor even after 3 days of "saturation". Can anybody help in choosing a...
24 July 2024 4,393 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View