I'am trying to prepare an electrode from GO-electrolyte gels, but the gels are coming out in the electrolyte solution , how can i solve this issue.?

01 January 1970 0 9K Report

I am trying to make an CV electrode using my GO-Electrolyte based gels mounted on glassy carbon. But after few minutes the mounted gel is coming out into the electrolyte solution (KOH). How can i solve this problem.?

Badges
Science topic

Similar topics
Gels

More Abhijeet Ojha's questions See All

What is the chemical effect of Graphene oxide cross-linking with metal ions?

I am exploring the effect of cross-linking between the graphene oxide nanosheets by mono and multivalent cations. How a metal ion reacts with GO nanosheets and what chemical changes it will...

08 September 2018 9,501 3 View

How can UV-Vis spectroscopy be used to calculate lateral dimension of 2D nanosheets?

I am trying to calculate the lateral dimensions of prepared graphene oxide nano-sheets dispersed in water, several articles have indicated that UV Vis can be used as a tool to do so . However, i...

01 February 2018 8,097 3 View

Is it possible that the surface functional groups of graphene oxide (GO) will be fully subsided due to the excessive ultrasonication?

I am studying the effect of extended ultrasonication on the synthesized graphene oxide nanosheets ( specially on the activity of surface oxy-functional groups ).

31 December 2017 8,987 6 View

How the polydispersity of graphene oxide nanosheets synthesized by hummer's process can be minimized ?

I am preparing the GO nanosheets using Hummer's process, but synthesized nanosheets are of very high polydispersity in terms of lateral dimensions. Is there any method by which the polydispersity...

11 December 2017 325 3 View

What are the suitable degassing conditions for Graphene Oxide and its derivative gels?

I have synthesized Graphene oxide based gels through cross-linking of GO nanosheets by metal ions of various valencies. What should be the optimum degassing conditions for its lyophilized form ?

10 November 2017 4,368 5 View

Is it possible to segregate graphene oxide nanosheets from a solution on the basis of their lateral dimensions?

I have an aqueous solution of exfoliated graphene oxide ( by ultrasonication) , Now if i wish to segregate these nanosheets on the basis of their lateral dimesions, how can i do so.?

09 October 2017 9,656 3 View

What is the effect of pH on aqueous dispersion of graphene oxide?

I am looking for the stability of aqueous dispersions of graphene oxide at various pH, can anyone describe me what should me my actual methodology to do so.? Also why these dispersions are most...

05 June 2015 4,214 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View