Hi Abhijeet,

Centrifugation,... different G force for different particle size. Low G-force will precipitate large particles at the bottom of the centrifuge tube. Take the supernatant suspension from the top and centrifuge again in a different tube with higher G-force. This will precipitate 2nd-large particles again but these will be smaller size than the 1st one. Depending on your centrifuge RPM and time you can apply increasing G-forces to precipitate decreasing particle sizes. You need to collect the precipitation only (use a dropper to take out the suspension from the top of the tube without disturbing the precipitation just after centrifugation) from each G-force to separately disperse them in different solutions....

Hope that will provide you some help..

Good luck...

CB

Dear Dr. Chandan

Thanks for your kind suggestions, in line with the present discussion i would like to know that how we can make a lateral size histogram with the segregated nano-sheets of different aspect ratios ? Since the number of nanosheets will be too large in the solution up-to what instant AFM will help in measuring dimensions.

Dear Abhijeet,

AFM will take too long... for making histogram, you need lots of data. So for making large statistical analysis, such as histogram by using AFM will take months... Its not efficient at all... Moreover, your particle size could be much larger (for low G-force) than the AFM scanning resolution..

What you can try like this..

This will give you a large no of data which you can put statistically between particle size and G-forces..

Good luck,...

CB..