18 August 2023 1 7K Report

Green synthesis of nanoparticles

More Afifa Israr's questions See All
How do I properly normalize spectra/peaks for comparison (Photoluminescence and Electroluminescence) ?

Hello, This questions pertains to comparing peaks from spectra of 2 or more different samples in a fair way through normalizing I know Origin normalizes based on the highest peak however if I...

12 December 2020 403 1 View

DNA Extraction from Monilinia fructicola (Fungi)?

I am doing DNA extraction using CTAB method from different fungal strains including monilinia spp. isolated from peach. My DNA concentration is very low. Is there any suggestion to improve the...

21 January 2019 8,673 3 View

Implementation of Dynamic contact angle in COMSOL?

Dear all I have been trying to model droplet dynamics over the solid surface when wettability gradient (Contact angle of the surface) is changed in COMSOL Multiphysics. However, the basic model...

23 July 2018 6,513 1 View

What is the best method to find the accurate optimum temperatre and pH and also find the thermal stability of wild type enzyme and its mutants ?

Hi ! I am finding the optimum temperature and pH of thermostable enzyme and its mutants. I find the optimum temperature of wild type enzyme and its mutants as 80C and 70C at 10 minutes...

11 December 2017 4,820 1 View

Direct couplin in ANSYS?

Hello everyone, does anybody know that how to couple Fluent and electric in ANSYS. I want to know the direct coupling procedure of these. It would be a great help. With warm regards

31 August 2016 2,422 2 View

How to get Heat flux at different time step for a transient natural convection in ANSYS Fluent?

I am doing research on the combined effect of natural convection and forced convection by providing velocity through ventilation ports in a rectangular enclosure keeping bottom plate heated at...

29 April 2016 5,456 6 View

Looking for research appoturnities regarding participation as an Author or co-author

Hello Respected All I'm looking for research opportunities as an Author or Co-Author for the research designs such as: Quasi-Experimental, Experimental, Mix Method, Systemic Reviews,...

01 January 1970 8,460 3 View

Can i use Disodium p-nitrophenyl phosphate Hexahydrate instead of Disoium p-nitroheny tetrahydrate?

I want to prepare PNP solution to analyze acid and alkaline phophatase activity in soil, and for p-nitropheny phosphate solution i could not find the Disodium p-nitropheny tetrahydrate

01 January 1970 4,132 2 View

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Ester cleavage conditions?

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01 March 2021 8,766 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to predict the reaction pathway for electro organic synthesis?

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