If you're doing kinase activity assays the old fashion way with radioactive labelled ATP and peptide substrate you might use P81 cation exchange chromatography paper to capture the peptide. Whatman, now GE Healtcare has stopped the production and I can't find anywhere to buy this P81 paper anymore. I figure that if everybody who is in need of this product contacts GE Healthcare and ask them to start production again, we might get them to do it. So please help.
Yep - became precious commodity few years ago. I had few rolls of it for a while, thankfully. But these days fewer and fewer people work with radioactivity I guess, so it's going the way of dodo and camera film...
This has been a big problem in my lab, with a long history in kinase assays. We have developed P81 production in our lab - results are consistent and indistinguishable from GE Healthcare. I am happy to dispense tester samples for validation if you email me your postal address: [email protected] On the back of larger orders, we will charge reasonable prices to cover costs, our motivation is to provide this essential resource to the wider scientific community.
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