I have RNA from some animals that I would like to identify. Are there any issues I should know about with using RNA rather than DNA for cytochrome c oxidase I (COI) sequencing?
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Hi, How do I interpret the p-values of the goodness-of-fit test results in ordinal logistic regression? I'm looking for literature in the internet but I couldn't find any. The tests that I need...
28 May 2024 6,554 2 View
Hi, I am trying to do an Ordinal Logistic Regression (OLR) in R since this is the regression analysis that I need to use for my research. I followed the tutorial video I found in YouTube in...
19 May 2024 4,660 4 View
Hi, I need help on how to increase the Cronbach's Alpha of my survey questionnaire. The survey questionnaire uses a 7-point likert scale and I had 12 respondents for its pilot testing. The...
16 May 2024 1,697 7 View
I want to measure correlations between human ratings of second language speech and a number of quantitative measures in the speech, such as articulation rate. I have collected speech rating data...
02 May 2024 6,400 0 View
Hello, As part of my statistical analysis for my thesis, I have done a number of one-way MANOVAs to look at whether there are between-group differences on a number of different...
28 April 2024 5,767 2 View
This question aims to understand the rationale behind the study, which likely sought to evaluate the comparability of gel separator serum and LIH plasma tubes in clinical chemistry and immunoassay...
09 April 2024 6,680 1 View
what are the challenges faced by international students on full time study concerning child care arrangement?
03 April 2024 5,556 0 View
Dear all, I am seeking to build a resource linking diseases with bioactive compounds, and include source (plant species) and range information as well. To make sure that I am not reinventing the...
29 March 2024 9,997 0 View
I am wondering about publishing a protocol for an interventional, prospective trial, but I am unsure about funding the trial. On one hand, I believe publishing a study protocol generates interest,...
27 March 2024 2,077 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View