I am transforming yeast grown on YPDA media and then plating them on SD/-Trp/-Leu. After obtaining the transformants, I dilute the colonies in water and plate on SD/-Trp/-Leu/-His /-Ade X-@ Gal media. While I am able to get the blue coloration on positive controls, the yeast does not seem to grow at all and cannot be photographed nor can infer on the interaction properly. It is just a blue translucent type growth. I use 20mg/ml x-@ Gal after autoclaving. The SD media is pH checked. Please give your opinions!