I induced arthritis to my mice strain. I would like to get some protocol or know how I could isolate cells from the joints and be able to do a FACS analyse and not just an histochemistry.
Thank you so much for your help.
Mithun Vishwanath K. Patil
this link will help you about the protocol for Fluorescence-activated cell sorting
http://www.pmf.colostate.edu/moflo_submission.html
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144656/
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Ishak Ozel Tekin
Dear Natalia,
You need synovia infiltrated cells. Before FACS analysis, you should handle single cell suspension from synovia by mechanical or enzymatically degradation method. You can use Medimachine device for mechanical degredation of tissues.
Good Luck
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