Natalia Bowakim

6 Questions 4 Answers 0 Followers

Questions related from Natalia Bowakim

When is the best time point for INF-g mRNA detection in T cells activated?

Hi, I would like to estimate the best time point to observe IFN-g mRNA in T cells activated with aCD3 stimulation. In this condition, I have already verified IFN-g protein production at 48h.

07 September 2016 7,519 2 View

Does anybody has / know how to get FoxP3-Cre mice SPF in Europe?

I need Foxp3-cre mice SPF. It's not available in Charles River Laboratories. I am wondering if any of you know how to get this mice strain avoiding JAX. Thank you all.

21 April 2016 1,021 3 View

How many times can I use a PD10 column?

When I use it several times the efficiency of the purification of my protein is not as much good as usually. Thanks for your advices

28 July 2015 2,703 4 View

How can I isolate and get a good purity of Treg cells in vitro?

I use FoxP3-GFP mice to sort Treg cells as GFP+. But once I plate my Tregs with CD3, CD28 and IL2 to maintain the survival of Treg cells and observe its effect in vitro, after 72h (normally due to...

04 February 2015 3,031 5 View

Isolation of T cells and DCs from joints (Arthritis model)?

I induced arthritis to my mice strain. I would like to get some protocol or know how I could isolate cells from the joints and be able to do a FACS analyse and not just an histochemistry. Thank...

13 October 2014 2,922 2 View

How to induce arthritis in C57BL/6?

Hi, can someone help me to find a model to induce arthritis in Black6 strain mice? I found that collagen-induced arthritis doesn't work properly in C57BL/6. Thank you so much

22 September 2014 6,226 6 View