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Questions related from Natalia Bowakim
Hi, I would like to estimate the best time point to observe IFN-g mRNA in T cells activated with aCD3 stimulation. In this condition, I have already verified IFN-g protein production at 48h.
07 September 2016 7,506 2 View
I need Foxp3-cre mice SPF. It's not available in Charles River Laboratories. I am wondering if any of you know how to get this mice strain avoiding JAX. Thank you all.
21 April 2016 1,016 3 View
When I use it several times the efficiency of the purification of my protein is not as much good as usually. Thanks for your advices
28 July 2015 2,700 4 View
I use FoxP3-GFP mice to sort Treg cells as GFP+. But once I plate my Tregs with CD3, CD28 and IL2 to maintain the survival of Treg cells and observe its effect in vitro, after 72h (normally due to...
04 February 2015 3,028 5 View