Hello, I am working on oxidative stress induction in cardiomyocytes using H2O2. I am using SOD2 as a marker for oxidative stress. I am going to treat H2O2 (150μM) for 12 hrs. Is using SOD2 as a marker for oxidative stress a good option. Any suggestions are invaluable.Thank you.
Hi, while SOD activity modulates oxidative stress, I would not consider it as an oxidative stress marker per se at least not as a direct marker. I have observed that SOD expression and activity may be regulated by exposure to H2O2 or superoxide anions. This regulation seems to be biphasic. As a very direct and straightforward marker in cardiomyocytes I would suggest 3-Nitrotyrosin or 8OH-dG which can both be determined by IHC or in tissue lysates by Western blot. ELISAs are also commercially available.
Dear sudheerji,
If you are measuring only SOD2 as a marker of oxidative stress, then it is not correct. You can use SOD2 with other relevant markers of oxidative stress such as lipid peroxidation products, DNA fragmentation products or oxidized proteins. I agree with Giancarlo Lopez-Martinez that peroxidase/catalase will be better than SOD2 with oxidative stress biomarkers for your work.
SOD2 can provide some useful information of oxidative stress. However, SOD2 increases under mild or moderate oxidative stress while decreases in responding severe or prolonged oxidative stress. Therefore, SOD2 alone can't be a reliable maker for oxidative stress.
Hi dear sudheer
I am working on oxidative stress in HUVEC cell .I treat huvec with 140uM h2o2 0.3% for 16h .
What percentage of hydrogen peroxide did you use ?
I recommend you this paper; I hope you find the protocol which you need,
you add this paper to your references.
cordially
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Medical Pharmacology
- Clinical Pharmacology