Is this method mentioned for sporulation assessment reliable ?

06 January 2021 1 10K Report

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under 10X in 3 microscopic fields and average number has been considered for scoring. Spore number is then analyzed as a scale based on spore number range.

Is this method reliable and can be used in all cases?

Otar Shainidze

Thank you so much for your cooperation.

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