Is this method mentioned for sporulation assessment reliable ?

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under...

05 January 2021 9,468 1 View

How to calculate width and length of the tapered microstrip line feed of a SIW leaky wave antenna?

With reference to substrate integrated waveguide -leaky wave antenna

26 December 2020 3,982 2 View

How can I extract radial stress from cartesian coordinate system in COMSOL?

Hello, I have a simulation in COMSOL which is a beam that is under bending and I don't know how could I extract radial stress of it from cartesian components. Is there anyone who may help me?

23 December 2020 8,408 2 View

Hi, EMG sensor value on aurdino is coming zero. Sometimes it works, sometimes it gives zero value. Anyone can help?

I have made all the connections properly, still, it is showing zero.

07 September 2020 3,313 4 View

Do the symptoms once appeared initially get disappeared towards the end of the season?

I am documenting disease incidence of leaf spot in different fields of eggplant. It was observed that DI% declines towards the end of cropping season. Plants are selected randomly. Do the symptoms...

31 July 2020 4,353 6 View

Among smartdraw, lucidchart and visio, which software is good to make diagrams in research papers?

For drawing any kind of charts and diagrams.

30 May 2020 8,496 6 View

Can someone guide me with Lactobacillus casei cell wall extract (LCWE) Perparation protocol?

I am preparing a Lactobacillus casei cell wall extract (LCWE) to generate a mouse model of Kawasaki disease. I have a query that some researchers go directly to a sonication step after overnight...

04 January 2020 2,470 1 View

Expression of multi disulfide bonds containing protein in bacterial system?

I have a insect protein which contains 4-6 disulfide bonds in its active form. I want to use a bacterial expression system (or yeast if it does not work out for bacteria) for over-expression of a...

12 June 2019 4,889 5 View

Is it possible to get amorphous nature from the SAED pattern ( diffused rings) and lattice fringes from the TEM image for the same sample?

For my sample, diffused rings are observed in SAED pattern which is due to the amorphous nature of the sample. In XRD also, amorphous nature is reflected by the presence of broad peaks. But, in...

10 June 2019 7,367 8 View

What do you think about the superiority of a paper published in a high impact factor SCI journal & a low impact factor SCI IEEE Transactions journal?

30 November 2018 4,365 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View