Good day,

I had just extracted a plasmid DNA from a 10ml of culture (pGEX construct, as I worried that the copy number would be too low, hence I decided to take 10ml for the DNA extraction). The expected size of the DNA is around 6Kbp. But as you can see, the gel results showed smearing and I have searched from the internet that tells me that this seems to be a highly concentrated DNA while some remarked that this is a degraded DNA. My lab doesn't have nanodrop or Qbit that could tell me the concentration of DNA.

Hence, I am here to ask anyone who has the experience to give some remark on the sample. I am using Thermo Fischer 1kb O'GeneRuler ladder for this gel.

Thank you in advance.