Is this a contamination?

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N-methyl-N'-nitro-N-nitrosoguanidine?

Hello, everyone We thinking of study with n-methyl-n'-nitro-n-nitrosoguanidine but I couldn't find solubility of n-methyl-n'-nitro-n-nitrosoguanidine. I just find that it can soluble in hot...

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Mixed cell line and a cytotoxicity assays?

For the work we'll do, we plan to use LLC cell line on mice but it is a mixed and we don't know which how we measure cytotoxicity in its. Does anyone have the answer???

09 October 2017 4,830 2 View

M30, M65 and murine cancer model

We wanna create lung cancer in mouse as part of a project but we worry about one matter. Are M30 and M65 assays practicable for murine blood sample?

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How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

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E.coli contamination in human RNA seq data ?

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Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

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Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Can't spin-orbit coupling(SOC) be included in optical properties calculation in CASTEP?

I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...

02 August 2024 2,890 0 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

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Adnan Ayhanci

yes, ıt's a viral contemination.

Özgün Teksoy

How is your decide to viral contamination?

Ron Reiserer

Unless you are noticing growth of colonies or morphological changes to your cells, it's most likely just dead cell debris.

Darren David Thomson

Its probably dirt on the glass. if it wriggles or swims around it may be bacteria!