Is there specific RFU value for different cDNA samples in qPCR ??

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Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

1.How can put nodes on equal distance in WSN? 2.How calculate retransmission of each node in WSN? 3.How to create buffer for each node?

I am using matlab simulator and doing paper selfish node detection based on average retransmission number. Some factors determine selfish node in wsn -If energy is not sufficient to forward...

28 December 2020 7,294 2 View

How to determine/select the indices (underlying weather index) of weather-based insurance?

It should not be confused with strike and limit thresholds. In this case, I am emphasizing the basis of the index, such as: -Cumulative, accumulated, deficit and excess rainfall values. -Average,...

29 November 2020 3,084 3 View

Can anyone help with SDS-PAGE problems?

i'm working on protein evaluation in flour of wheat and others legumes.....I'm using SDS-PAGE. However those days (about two weeks ago) i foudd nothing in the gels (no bands), i dont know what's...

11 November 2020 2,330 3 View

Why can`t I isolate RNA from Extracellular vesicles?

Hello, I have been trying to isolate RNA from EVs (from MSCs) for a while. I isolate the EVs using SEC. Then I use the "Total Exosome RNA and Protein Isolation Kit" from Invitrogen. When I...

21 October 2020 8,559 4 View

Do you know someone with knowledge of France in 1700s?

I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...

01 October 2020 9,095 5 View

Can megakaryocytes be analyzed on flowcytometry?

Hi there Can anybody share their experience with flowcytrometric analysis of megakaryocytes in bone marrow samples? Types of markers you used, any set-ups you applied and also whether you used...

01 July 2020 5,676 3 View

How to directly use a directory of .h5 files as the training set in Python without appending them to a list?

I am trying to append .h5 files saved in a directory for my training set using Python, but I face memory error as the Data set is very large. The following code is what I used for...

23 April 2020 9,649 2 View

What is the best way to remove bacterial contamination in my mammalian cell sample?

I have a two part question: (1) If DNA is extracted from a mammalian cell and the total quantity (yield) attained is significantly larger than what is theoretically expected considering the total...

25 March 2020 4,044 3 View

Not sure if some of the entities I am looking at inside my hemocytometer are debris from the k562 cells or bacteria which contaminated my sample?

I am conducting DNA extraction experiments from K562 cells. For a reason unbeknown to me, the total mass of DNA extracted happens to be much larger than what I expect considering the number of...

15 March 2020 6,223 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View