without use trypan blue
Hi ... yes there is anothor method but trypan blue is the easier one
Viability was measured using both SYBR-Green I, which penetrates live and dead oocytes (green fluorescence, 500-530 nm) and propidium
iodide, which penetrates only cells with compromised membrane and then emits in the red fluorescence range (550-600 nm).
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion
25 July 2024 2,486 2 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
In your opinion, I would like to know if a qualitative study with a structured interview can be conducted in writing. In other words, we sent out open questions and asked the interviewees to...
11 July 2024 4,163 4 View
What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?
08 July 2024 7,464 1 View
How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...
08 July 2024 6,609 2 View
what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?
08 July 2024 7,869 1 View
Where can I download Bergey's Manual of Determinative Bacteriology?
27 June 2024 8,055 2 View
In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...
26 June 2024 6,941 3 View
Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...
23 June 2024 4,763 0 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View
Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...
10 July 2024 640 1 View
Hematoxylin, DAPI, Methylene blue, Toluene, etc.
23 June 2024 894 3 View
methylene blue stock solution
19 June 2024 10,030 3 View
I intend to have my anti-reflection coated window at roughly 20° angle of incidence the question is if it red shifts or blue shifts in terms of transmitted wavelength
30 May 2024 2,589 2 View
what is the optical band gap of pure cobalt oxide nanoparticles? What is the reason for the blue shift observed in the UV-visible spectrum?
26 May 2024 8,219 2 View
I have cultured PBMC and some RBC contaminant for 2 weeks in suspension and low attachment plate. From the very first day, I noticed that there are many cells that have already turned all...
03 May 2024 1,000 7 View
Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...
02 May 2024 895 2 View
We do biosensor research and I was wondering if a short single-stranded DNA base pair of 50 is tagged with methylene blue at one end (5' end) and fluorescein on the other end (3' end), will it...
27 April 2024 6,459 0 View
Hi there, I am using 0.4% trypan blue dye to count my nuclei suspension (generated from snap-frozen intestinal colon tissue). Although I expected a blue stain only (since nuclei resemble the dead...
17 April 2024 4,472 0 View