What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

Can Vibrio campbellii bioluminescence be obtained when cultured on Mueller-Hinton agar?

Need to check quorum sensing activity of Vibrio campbellii.

20 January 2021 570 5 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

How long is it possible to store the bacterial culture at 4C?

How long is it possible to store the bacterial culture at 4C?

02 December 2020 2,750 11 View

Smear bands during colony PCR to identify mi gene of interest? but one positive?

28 October 2020 3,324 4 View

Can we conjugate rabies virus glycoprotein (RVG) directly with exosome?

I have read many articles where researchers get RVG-Exo by transfecting the exosome producing cell through RVG Plasmid. I wanted to ask if anybody knows, can we decorate exosome with RVG directly...

20 October 2020 2,365 2 View

Which tool for operon prediction in bacterial genomes ?

Hi, I want to predict operons on entire bacterial genomes (already annotated). I used to use operon-mapper ( https://academic.oup.com/bioinformatics/article/34/23/4118/5040321), which is great but...

07 October 2020 7,192 7 View

Anyone with experience on DNA extraction from biopsies for microbiome analysis?

We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...

24 September 2020 6,845 4 View

Asides using ethidium bromide, what other methods do we use for plasmid curing?

Is there any other substance, either radioactive or not, that can be used for plasmid curing? And what other methods?

23 August 2020 5,254 3 View