Hi there. If oxygen is not toxic to your bacteria and they are not heat-sensitive, you can add another agar layer after spreading/striking the plate. Or you may use "pour plate" to culture your bacteria.
Hope it works for you.
Dear Moon Baglari,
The old-fashioned way was to incubate plates in a sealed glass jar and a lit candle – the candle will burn briefly after the lid is put in place, removing oxygen. This works well if you are careful and so long as the CO2 produced is not a concern (it could effect the pH of solutions / plates).
Andrew
Dear Moon Baglari
Hi
Culture remains the gold standard for the identification and speciation of anaerobic bacteria .but a deluge of chemicals, reagents, media and equipment are commercially available for anaerobic study
Studying LABORATORY METHODS IN
ANAEROBIC BACTERIOLOGY
CDC LABORATORY MANUAL will help you more
I think this area focuses more on microbiology which is not my stronghold though have worked with a few in this area
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