I want to infect mammalian cells with a bacterial cell density of 10^5 and seed it either in 24 or 6 well plates. or anything relating to MOI
Hello Moon Baglari
You may refer to the research article attached below. You may have a look at the protocol Step 3.3.
Article In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Hope this may help!
Good Luck.
Dear Moon Baglari . See the following useful link: https://sciencing.com/calculate-amount-bacteria-present-8469410.html
Kindly see also the following useful link: https://assets.fishersci.com/TFS-Assets/CAD/posters/nanodroptryPOSTER.pdf
Also check please the following useful RG link: Article Mammalian cell culture test material generation for the asse...
The following RG link is also very useful: Article In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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