Is there is possibility of methylation of mature miRNAs?

09 September 2013 0 9K Report

I was wondering if anybody could tell me the possibility of methylation of mature miRNAs and If yes, how can we find it?

Badges
Science topic

More Sultan Asad's questions See All

Validation of circular RNAs?

Which method is more suitable for validation of circular RNAs; PCR or Northern blot? Can anyone suggest me the protocol of designing divergent primers for circular RNAs.

08 September 2017 4,901 1 View

Can someone suggest a suitable Insect Expression vector?

I want to express GFP protein in both sf9 and Aag2 insect cell line. We have cloned GFP protein in PIZ/V5-His insect expression vector. I got a very good GFP expression in sf9 cells (western...

09 October 2013 7,614 1 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Kraft lignin and MMA(Methyl meth acrylate) free radical polymerization for synthesis of thermoplastics?

I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...

15 July 2024 9,827 1 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View

What are some other proteins apart from histones that can be acetylated and methylated?

If they can be acetylated and methylated what will be the effect?

02 July 2024 4,030 2 View

To prepare polymethyl methacrylate polymer for the study of acoustic properties without of bubbles during this process, what measures should be taken?

To prepare polymethyl methacrylate polymer for the study of acoustic properties, a mixture of methyl methacrylate monomer and benzoyl peroxide is used. This mixture is then heated and placed on a...

27 June 2024 2,087 1 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View