Validation of circular RNAs?

09 September 2017 1 5K Report

Which method is more suitable for validation of circular RNAs; PCR or Northern blot? Can anyone suggest me the protocol of designing divergent primers for circular RNAs.

Sandeep RAJ R

cell free RNA once you isolate and convert it to cDNA then u can use normal PCR to validate only thing to take care is keep same primers will incrementing serial concentration of cDNA template and find optimal vol for getting positive band

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Science method

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