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Which method is more suitable for validation of circular RNAs; PCR or Northern blot? Can anyone suggest me the protocol of designing divergent primers for circular RNAs.
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I want to express GFP protein in both sf9 and Aag2 insect cell line. We have cloned GFP protein in PIZ/V5-His insect expression vector. I got a very good GFP expression in sf9 cells (western...
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I was wondering if anybody could tell me the possibility of methylation of mature miRNAs and If yes, how can we find it?
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