The cleavge of a glycosidic bond is very hard, so please let me know if there is any special enzyme which can do it in the case of glycolipids.
I would begin by trying any available Beta-glucosidases - commercial or otherwise. SigmaAldrich sells Almond B-glucosidase, for example. There is some literature supporting their use in hydrolyzing glycoeramides from membranes (eg. http://www.ncbi.nlm.nih.gov/pubmed/15669767). If access to commercial products is limiting, you could express your own - many have been cloned, functionally characterized and reported in the literature. Just ask the PIs for the clones - many are happy to accommodate. I unfortunately don't have any.
If the enzymes you try don't work, or if you don't want to wait for them to arrive, you could attempt to acid hydrolyze your molecules with a strong organic acid. I've prepared certain aglycone derivatives of alkaloids using this strategy.
Thank you very much for your answer Dylan.
Hopefull Beta glucosidase doesn't work in my case. Acid hydrolysis causes isomerisation that makes more compex during isolation.
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