I am using abcam MPO assay kit. They says in the web OD is inversely related to MPO activity. Does anyone explain how it could be? It seems directly proportional from my calculations.
I would like to know the gene responsible for synthesis of phytosphingosine not phytoceramide (des2) in human epithelial cells. If anyone have this information please share with me.
09 October 2017 5,590 0 View
Please assist me with the help of the reference attached.
07 August 2017 649 4 View
I would like to study the enzyme substrate interactions. But I could not find the protein structure of almond ß-glucosidase in the literature. If anbody have any idea concerning its structure...
01 February 2016 5,728 5 View
I am trying to establish adriamycin-resistant MCF-7 from normal MCF-7 by sequential treatment of Adriamycin, but until now not succeeded in establishing it. Everytime results will fluctuate and...
08 September 2015 5,711 4 View
I am experiencing very low O.D values in MTT assay, when I measured using microplate reader at 570 nm, even though I took enough no of cells there is no much increase in O.D. is it quite common or...
07 August 2015 2,945 13 View
I would like to conduct a transglycosylation reaction using ß-glucosidase, my both reactants are water soluble, in such case only buffer is enough to carried out the reaction. But if we use buffer...
03 April 2015 6,557 3 View
The cleavge of a glycosidic bond is very hard, so please let me know if there is any special enzyme which can do it in the case of glycolipids.
03 April 2014 3,298 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....
05 July 2024 5,860 1 View