Does OD is directly proportional to Myelonperoxidase activity in MPO assay by colorimetry?

I am using abcam MPO assay kit. They says in the web OD is inversely related to MPO activity. Does anyone explain how it could be? It seems directly proportional from my calculations.

09 October 2018 8,880 0 View

Does anybody know the interpretation of IR spectrum of Merrifield resin. Why always there is a broad band around 3400 cm-1?

Please assist me with the help of the reference attached.

07 August 2017 569 4 View

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01 February 2016 5,630 5 View

Why it is very difficult to establish Adriamycin-resistant MCF-7?

08 September 2015 5,630 4 View

What is the O.D range in an MTT assay ?

I am experiencing very low O.D values in MTT assay, when I measured using microplate reader at 570 nm, even though I took enough no of cells there is no much increase in O.D. is it quite common or...

07 August 2015 2,652 13 View

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03 April 2015 6,484 3 View

Is there is any enzyme to hydrolyze the beta glycosidic bond of Glycoceramides?

The cleavge of a glycosidic bond is very hard, so please let me know if there is any special enzyme which can do it in the case of glycolipids.

03 April 2014 3,217 2 View

No title

02 March 2021 3,060 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View