My glycerol stock bacteria does not grow?

I made 30 bacteria transformed with plasmid including ampicillin resistant gene. transformed bacteria (total 30) were mixed with 25% glycerol and stored in -70 defreezer ----> stock : 500 ul...

04 May 2019 3,118 2 View

How does a filamentous fungi get foreign genes?

In transformation experiment of M. oryzae, when I mix any genes with m. oryzae protoplasts, they are easily transformed by the genes. I want to know how they get foreign genes. Do they get the...

01 February 2016 5,065 1 View

Do you know about Fusarium sexual stage and asexual stage?

31 December 2015 9,348 1 View

In fungi, where are the proximal hyphal compartment and distal hyphal compartment?

08 September 2015 6,613 1 View

Any advice on the AFLP amplification step?

05 June 2015 6,501 6 View

Any advice on a white pellet in the lysis solution?

05 June 2015 2,413 3 View

What is degenerate cell?

Hello. I am reading a book that deals with virus evolution. In hypotheses on the origin of viruses, there is a sentence " Viruses are derived from degenerate cells that eventually parasitised...

04 May 2015 348 1 View

How can I make a 50pmol/ul EcoRI adaptor?

03 April 2015 8,316 1 View

How can I solve my protoplast generation problem?

01 February 2015 3,057 2 View

How long should one store neutral red dye?

31 December 2014 8,785 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View