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Questions related from Jong-hwan Shin
I made 30 bacteria transformed with plasmid including ampicillin resistant gene. transformed bacteria (total 30) were mixed with 25% glycerol and stored in -70 defreezer ----> stock : 500 ul...
05 May 2019 3,209 2 View
Hello Recently, my interesting is cell structure of red pepper. With my light microscope, I want to observe how Colletotrichum acutatum infect red pepper. I cut pepper fruit surface with a...
11 November 2016 7,628 0 View
In transformation experiment of M. oryzae, when I mix any genes with m. oryzae protoplasts, they are easily transformed by the genes. I want to know how they get foreign genes. Do they get the...
02 February 2016 5,168 1 View
On PDA, Fusarium grows as asexual stage. I heard that Fusarium grows as sexual stage on V-8 juice agar. I want to make my Fusarium isolates grow as sexual stage. Do the Fusarium isolates grow as...
01 January 2016 9,413 1 View
I think that "proximal" is "near" and distal is "far", but the figure I have attached say that " Vacuoles in ‘empty’ distal compartments of a hypha (black arrow heads) and cytoplasmic proximal...
09 September 2015 6,684 1 View
Hello! I want to know the genetic variability of C. acutatum collected from Korea So, I am working on the AFLP analysis of C. acutatum. first, I conducted the pre-amplification step and...
06 June 2015 6,593 6 View
hello . I have a lysis solution containing RNaseA for dna extractoin. I have stored the solution in a refrigerator for a long time. For that reason, the solution now shows some white pellets. How...
06 June 2015 2,558 3 View
Hello. I am reading a book that deals with virus evolution. In hypotheses on the origin of viruses, there is a sentence " Viruses are derived from degenerate cells that eventually parasitised...
05 May 2015 419 1 View
Hello. I am working on the AFLP analysis of C. acutatum fungi. I need to make EcoRI adaptor ( 50pmol/ul ). I have primer 1 and primer 2 that are somewhat complementary to each other to generate...
04 April 2015 8,393 1 View
Hello. I am working on the generation of C. acutatum protoplast from young mycelia. I used drisease as a lysing enzyme in 1 M NH4Cl. It seems to work well, but the protoplasts generated tend to...
02 February 2015 3,132 2 View
Hello. I am studying on the vacuole development of M. oryzae. I first used neutral red dye. I diluted the dye in phosphate buffer pH 7.4 It stained vacuole well, but It does not work well after...
01 January 2015 8,861 3 View
I have a insert in my vector. The size of my insert is approximately 6.7 kbp. When I sequenced the insert, I found 3 error sequences. I want to replace the error sequences with correct...
12 December 2014 4,286 9 View
I have made a gfp fusion gene. When I saw the gfp signal under a fluorescence microscope there were much gfp background signal. The gfp gene follows my gene (promoter-my gene-gfp...
12 December 2014 2,860 5 View
I need to generate Colletotrichum acutatum protoplast. I want to make the protoplast from mycelia. When I incubated the mycelia in PDB (potato dextrose media), the mycelia produced conidia. I do...
12 December 2014 2,469 2 View
