Is there anybody working on the oxygen mass transfer in cell cultures in a stirred tank bioreactor?

I have been working on cell cultures in stirred tank reactors. I want to know how to calculate the kLa (oxygen mass transfer coefficient) in such cases where we are growing cells inside the...

09 October 2018 2,157 5 View

How to improve the mesh quality of a stirred tank reactor?

I am simulating a stirred tank reactor using CFD. I meshed it, but the mesh quality is not good. How can I improve it. Can any one please help.

03 April 2018 9,543 4 View

How do we calculate the kLa for the cell culture, while changing the agitation rate inside the reactor?

Suppose we are growing the cells inside the reactor, we start with a fresh inoculum. How can we calculate the kLa inside the reactor, while changing the agitation rate (RPM). Ca anyone please...

02 March 2018 1,057 2 View

How can one exactly calculate the volumetric mass transfer coefficient in a stirred tank bioreactor once cells start growing inside the reactor?

I am currently working on hydrodynamics of stirred tank reactors for cell cultures and we are calculating the kLa and I want to know how can we exactly calculate kLa once cells start growing...

02 March 2018 6,906 46 View

What is the best method of determining the volumetric mass transfer coefficient while the cells are growing inside the reactor?

I am working on the E.coli cell culture trying to study the impact of rheology on the mass transfer coefficient in a stirred tank reactor. What is the best method of determining the kLa at...

01 February 2018 4,491 5 View

Is there anybody working on the CFD simulation of stirred tank reactors? Please let me know?

I am actually simulating the stirred tank reactor using CFD. I am using the Ansys Fluent to carry out the simulations. I am able to carry out the simulations for the single phase (water). However,...

11 December 2017 5,155 11 View

Is there anyone working on the Rheology of the cell cultures. Please let me know?

Anybody working in this field

11 December 2017 4,107 7 View

Can anyone tell me where from I can get the Admixtus Software used for simulating Stirred tanks?

I need this software for carrying out the simulation study.

11 December 2017 1,827 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View