Is there anyone working on the Rheology of the cell cultures. Please let me know?

12 December 2017 0 10K Report

Anyone working in this field

Badges
Science topic

More Sohail Lone's questions See All

Is there anybody working on the oxygen mass transfer in cell cultures in a stirred tank bioreactor?

I have been working on cell cultures in stirred tank reactors. I want to know how to calculate the kLa (oxygen mass transfer coefficient) in such cases where we are growing cells inside the...

09 October 2018 2,295 5 View

How to improve the mesh quality of a stirred tank reactor?

I am simulating a stirred tank reactor using CFD. I meshed it, but the mesh quality is not good. How can I improve it. Can any one please help.

03 April 2018 9,673 4 View

How do we calculate the kLa for the cell culture, while changing the agitation rate inside the reactor?

Suppose we are growing the cells inside the reactor, we start with a fresh inoculum. How can we calculate the kLa inside the reactor, while changing the agitation rate (RPM). Ca anyone please...

02 March 2018 1,471 2 View

How can one exactly calculate the volumetric mass transfer coefficient in a stirred tank bioreactor once cells start growing inside the reactor?

I am currently working on hydrodynamics of stirred tank reactors for cell cultures and we are calculating the kLa and I want to know how can we exactly calculate kLa once cells start growing...

02 March 2018 7,042 46 View

What is the best method of determining the volumetric mass transfer coefficient while the cells are growing inside the reactor?

I am working on the E.coli cell culture trying to study the impact of rheology on the mass transfer coefficient in a stirred tank reactor. What is the best method of determining the kLa at...

01 February 2018 4,678 5 View

Is there anyone working on the Rheology of the cell cultures. Please let me know?

Anybody working in this field

11 December 2017 4,215 7 View

Is there anybody working on the CFD simulation of stirred tank reactors? Please let me know?

I am actually simulating the stirred tank reactor using CFD. I am using the Ansys Fluent to carry out the simulations. I am able to carry out the simulations for the single phase (water). However,...

11 December 2017 5,305 11 View

Can anyone tell me where from I can get the Admixtus Software used for simulating Stirred tanks?

I need this software for carrying out the simulation study.

11 December 2017 1,965 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View