I stained the yeast cells with DAPI and Tunel and I need merge the two images. But I haven't software to do it. Is there anyone to advise me a software program to merge the images?
Merhaba. I would open the two images in Photophsop as JPEG or TIFF files. Once in Photoshop you can superimpose the two images as two layers. This would let you play with the transparency of each layer and adjust contrast and gamma of the two layers separately.
If you ultimately need to segment the two images, perhaps in an effort to find nuclei in one image within cells of the other, I'd strongly recommend you take a look at MIPAR at http://MIPAR.us. I'd happily make an automate recipe file for you and send it over!