Hi, everybody
I am working on phytase production by a B. subtilis strain. We are going to scale up the production using a NBS BioFlo 310 Bioreactor. We are going to use NBS EX-2000 Gas Analyzer for measuring O2/CO2 from the bioreactor exhaust, but unfortunately, we are having difficulty working with this equipment. Although, the air enters and exits from the machine, but its flow-meter doesn't work and the results aren't satisfactory! Also, how can I make sure that the machine is working properly?
Thank you in advance!
check it with standartds of gases, O2 pure or a sample of mix (CO2 O2 and N2)
Hello Karim.
I would contact the manufacturer (Eppendorf) and ask for advice.
Perhaps your exhaust gas flowrate is out of range (0.5 to 1.0 L/min) or the pressure is too high (0.5psig max).
I would also calibrate it before each use, you can buy specific gas mixtures from compressed gas suppliers.
Good luck.
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