Is there any way to validate NEB stable cells using PCR?

More Assim Verma's questions See All

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How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

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How to adjust a covariate in meta-analysis?

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11 January 2021 9,409 4 View

Is it possible that first waiting time decreases and then after a certain point waiting time start increasing?

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22 December 2020 2,455 1 View

Which gas is used for Bunsen burner in ASTM D6413 Vertical Flame Test?

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09 November 2020 1,808 1 View

How does culture impact our ability to learn?

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14 October 2020 9,107 7 View

How to produce ROC/AUC curve using various groundwater vulnerability method for comparative analysis?

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When to add H2DCFDA dye before or after treatment and why?

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Poisson's ratio of nano hydroxyapatite?

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How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

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01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

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What is the ideal efficiency for a qPCR?

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28 February 2021 1,254 3 View

Mahmudul Hasan

Assim Verma you are trying to confirm a transformation or just the bacteria itself!

Hanna Alalam

You don't need PCR to verify. NEB stable carries an F' plasmid that has a tetracycline resistance marker and is also streptomycin resistance. You can substreak your strain on LB with tetracycline and streptomycin to validate and that is likely to be enough.