If I can use normal mice, how can I suppress the immune system?
This is a very tricky experiment to do xenograft with "normal" mice. I agree with Chris, you never know how efficient immunosuppressive treatments will be and how it will affect the xenograft transplant results. Be careful from the beginning, your reviewers will be very critical with this experimental setup. You should consider a "clean" xenograft model with NOD/SCID or similar models. There are huge blogs on how to use matrigel on Researchgate. Please also check these blogs. Most important is to keep the matrigel and all tools and solutions cold at 4°C. Don´t dilute the MG higher than 1:3 with cells and inject 100-200 µl in the mammary fat pad or s.c. Good luck.
Hello,
For xenograft studies,making normal mice to immunocompromised is not at all an easy task..Even after immunosuppressive treatment you wont be sure about complete immune blockage. Partial Immune laekage is commonly seen Even after successive generations in athymic NUDE or Nu/Nu mice because they carry immature B cells.SCID are considered as a proper immunocompromised mice.
Keep these points in mind before planning for any experiment.
We use Standard HC BD Matrigel matrix with out any dilution with cells in 1:1 ratio.Total injection vol. should be 0.2 ml.
Hope these informations will be helpful for you.
best luck..
milind
A lot of thanks for all of dear and kind research friends.
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