Do you want to inject the lysate or add it to the culture medium?

Markus Thank you for interst.... I want to add the injured brain lysate to imortalized cell line to check the spreading activities of the stated markers in cell culture.....

Regards

The high fat content of the brain lysate can kill cells in culture. You could try to the soluble fraction of the lysate first. After centrifugation >20000g  for min. 15min remove the insoluble pellet and the fatty overlay. If this fraction is active, you can fractionate further ( for proteins etc.). If not you could try the soluble fraction mixed with the pellet. You could also think of separating grey and white matter, grey matter reduces the problem.