Shafiq Ur Rehman

6 Questions 8 Answers 0 Followers

Questions related from Shafiq Ur Rehman

Is there any way to transfer the injured mouse brain lysate in immortalized cell line to check the spreading activity of stress markers?

I am working on traumatic brain injury in mouse model.. I want to transfer the "injured brain lysate" in the cell culture to check the spreading activity of stress marker in cell line......

08 January 2016 5,511 3 View

Can I use BV2 microglial cell instead of rat/mice primary microglial brain cells ?

In most studies the primary microglial cells of the rat brain are use for experiments and I have the BV2 cells ,, So can I use the BV2 cells instead of primary microglial cells of the rat pups brain?

13 April 2015 7,531 6 View

Is there any simple protocol for the isolation of mouse hypothalamic neuronal cells?

Is there any simple method for the isolating and possible protection of the hypothalamic neuronal cells from mice ?

11 March 2015 8,480 8 View

Is there any procedure for the direct attachment of polyphenoles/Anthocyanins to PEG-gold nanoparticles?

Hi, is there any simple method to attache anthocyanins/ hydrophilic compounds to PEG-gold nano particles?

16 July 2014 2,071 1 View

Does anyone have an idea about how to use normal serum in double immunofluorescence staining?

I used normal Rabbit serum in a blocking solution,then attached anti-goat primary antibody, next day used Rabbit anti-goat FITC, second primary antibody was used anti-Rabbit, on 3rd day used...

03 April 2014 852 0 View

How can I calculate the concentration of Gold nanoparticles for in-vitro study in terms of nM or micromole?

I have Gold nanoparticles (5nm)-PEG coated. OD (50) and the total vol. is ml. How can I calculate the concentration of Gold nanoparticles for in-vitro study in terms of mM of micromolar?

11 March 2014 1,275 9 View