Is there any way to ship cell lysates (for western and Co-IP) abroad?

More Mohamed Kamal's questions See All

What kit or method is suitable for the extraction of RNA from around 50 cells?

Dear All, I am wondering if you could recommend a good kit or method for the extraction of RNA from as low as 50 cells. I need to run RNA seq afterwords. Looking forward to your...

31 December 2017 1,411 2 View

Could a tumour suppressor gene enhance the activation of PI3K pathways?

I work on a tumour suppressor gene in breast cancer. The down regulation of this gene in MCF10A cells (using shRNA) caused a 12 folds decrease in the mRNA expression of PI3K catalytic subunit...

31 December 2013 896 8 View

Do I have to confirm the qPCR array data using specific qPCR assays?

I ran qPCR arrays to test the expression of 48 genes in two samples. 10 genes showed significant difference between samples. The assay was very reproducible (nearly same results between...

09 October 2013 3,713 8 View

Do I have to confirm the qPCR array (Stell Array, Lonza) data using specific qPCR assays before testing the protein expression of these genes?

I have used qPCR arrays to test the expression of 48b genes in two groups of cells. These arrays showed 10 genes which their expressions are significantly different between the groups. The next...

08 September 2013 7,087 2 View

What are the best ways for selecting genes to be included in qPCR arrays?

I am currently designing qPCR array to investigate the effect of my gene of interest (CSMD1) on the expression of a number of genes. My cell culture studies showed that CSMD1 affects cell shape,...

04 May 2012 1,946 7 View

Does anyone tried mammosphere assay for circulating tumour cells?

01 January 1970 5,309 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Kwok Leung Ong

There is a global shipping company, called "World Courier". Our institute always ship frozen samples to oversea countries using this company.

Matteo Da Ros

The lysates must be shipped in dry-ice, otherwise your proteins will get degraded. There are different companies (we usually use DHL or FedEx) that accept dry-ice shipments. You should ask their customer service.