What kit or method is suitable for the extraction of RNA from around 50 cells?

Dear All, I am wondering if you could recommend a good kit or method for the extraction of RNA from as low as 50 cells. I need to run RNA seq afterwords. Looking forward to your...

31 December 2017 1,411 2 View

Could a tumour suppressor gene enhance the activation of PI3K pathways?

I work on a tumour suppressor gene in breast cancer. The down regulation of this gene in MCF10A cells (using shRNA) caused a 12 folds decrease in the mRNA expression of PI3K catalytic subunit...

31 December 2013 896 8 View

Do I have to confirm the qPCR array data using specific qPCR assays?

I ran qPCR arrays to test the expression of 48 genes in two samples. 10 genes showed significant difference between samples. The assay was very reproducible (nearly same results between...

09 October 2013 3,713 8 View

Do I have to confirm the qPCR array (Stell Array, Lonza) data using specific qPCR assays before testing the protein expression of these genes?

I have used qPCR arrays to test the expression of 48b genes in two groups of cells. These arrays showed 10 genes which their expressions are significantly different between the groups. The next...

08 September 2013 7,087 2 View

Is there any way to ship cell lysates (for western and Co-IP) abroad?

The next step of my project is to do western blotting and Co-IP. However, the cells which I will use are not in my lab, they are in my collaborator lab abroad. Because I do not have cell culture...

11 December 2012 4,739 2 View

What are the best ways for selecting genes to be included in qPCR arrays?

I am currently designing qPCR array to investigate the effect of my gene of interest (CSMD1) on the expression of a number of genes. My cell culture studies showed that CSMD1 affects cell shape,...

04 May 2012 1,946 7 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View