Does anyone tried mammosphere assay for circulating tumour cells?

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Valentine Comaills

Hi,

Do you want to culture primary CTC form patient or mice? mice 's ones are relatively easy but from patient it is more challenging....

here you have a beautiful paper :-P about it!

https://www.ncbi.nlm.nih.gov/pubmed/25013076

good luck!

Abdul Mannan Baig

Like prostatosphere, yes it is possible. Try the patient's CTC into sphere in RPMI and DMEM, both with Insulin added.

Best wishes,

Mannan

Mohamed Kamal

Yes indeed I want to do the mammosphere forming assay from patients CTCS. I am currently trying the media described in the paper you sent Valentin but I am not sure if it's gonna work for cells cultured individually.