I have contaminated HEp2 cells -how should I decontaminate them? is there any way?
Difficult. First determine if it's bacteria or fugi. Try use 0.1 mg/ml ampicillin in your medium in bacterial contamination and change medium every day for three days in a roll. Good luck.
28 February 2021 5,433 3 View
I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...
27 February 2021 4,218 7 View
Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...
21 February 2021 4,453 3 View
Could I please get the guidance on Helix antenna operated from 1.5 - 1.8 GHz. I am designing in hfss and not able to cover 1.5 GHz. Could I get papers or suggestions on numbers I use to cover this...
02 February 2021 622 7 View
Should I make 2 antennas, one for 2.4 GHz and one for 5.8 GHz? How should I approach the problem if I want to design the antennas which cover 400 and 900 MHz and 2.4 and 5.8 GHz? All together in...
26 January 2021 906 3 View
So, I have a design, two dipoles working at UHF and VHF. We fed using a diplexer. I am having difficulties with the testing using network analyzer. VHF passes but UHF, specially at 400 MHZ, the...
26 January 2021 8,743 8 View
I have a design with 2 dipoles working at different frequencies joined in one system and separated by Ferrite. I think, Ferrite was used to minimize the interference between 2 antennas as much as...
25 January 2021 5,918 3 View
The solubility of L-tryptophan is 11.4g/l in water. I tried dissolving 1.14 g in 100 ml PBS but still, it's not fully dissolved. Should I add NaOH or HCl? Also, can we stir the solution, or...
22 January 2021 1,127 3 View
So, I came across, where antennas are being tested in a building ( a plant), where all sorts of big equipment are around and of course the ceiling. lot of multipath which can affect the...
21 January 2021 9,437 3 View
Helical antenna usually have fixed spacing between the coils. What is the advantage of varying the spacing between the helical antennas? Varying the pitch angle. Thank you for your guidance in...
21 January 2021 8,864 4 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View