I have contaminated HEp2 cells -how should I decontaminate them? is there any way?
Difficult. First determine if it's bacteria or fugi. Try use 0.1 mg/ml ampicillin in your medium in bacterial contamination and change medium every day for three days in a roll. Good luck.
Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
11 June 2024 8,915 0 View
I have isolated granulosa cells from mice ovary using puncture method. Cells are healthy and growing in DMEM. Their morphology is same as granulosa cells. However I would like to use a molecular...
12 May 2024 3,890 3 View
because people are citing/talking less about Classical texts discovered by Rishi/sages. Rishi has the broad scientific knowledge as per their practical and theoretical practices.
30 March 2024 2,013 0 View
I am working on T cell receptor repertoire analysis obtained from RNA seq data. I am trying to understand the CDR3 length and amino acid usage in my data set, where I have variable lengths of...
12 March 2024 3,000 0 View
There is usually airgap between a radiating element and metasurface and I want to calculate the phase delay analytically. Like what is the reflecting phase from the meta? What is the phase delay...
06 March 2024 7,187 1 View
is there any model or formula to calculate the length of the partial ground plane? thanks in advanced
19 January 2024 5,022 3 View
I am in the beginning phase of antenna design and i am confused regarding the shape of the radiator.Thanks in advanced
14 January 2024 8,761 3 View
While running HWRF model, we get the GRIB1 files with extension 'egrb' which contains all the variables. How can we convert this file to nc? I have tried using cdo and wgrib but got errors due to...
01 January 2024 5,238 2 View
The number of pores in the R.10 flow cell decreased significantly from 1500 to 500 after nanopore sequencing with only 24 samples multiplexing. I'm using the SQK-NBD114.24 kit as the standard for...
11 December 2023 7,408 0 View
In industrial PECVD system, anyone face burn-mark issues on final wafer?
15 November 2023 6,934 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View