How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

02 December 2020 9,424 5 View

Why CD4 Treg lose FoxP3 during expansion in vitro?

Hello everyone! I have the following question: I tried to expand CD4 Tregs in vitro to get enough material for WB or any other application. This is my first trial with them. So, I`ve got FoxP3+...

13 September 2020 6,458 6 View

How should I care of mice after sublethal irradiation?

Hello dear colleagues I plan to test the primary antiviral response of CD8 T-cells of different genotypes, WT and with total knockout of some gene. The problem is that the KO mice that I plan to...

22 July 2019 1,844 3 View

Do exist any approved to human (or, at least, near to be) anti-inflammatory drugs, alternatives to NSAIDs?

09 August 2017 2,919 7 View

Classifier robust against non-informative features?

I have two feature sets I evaluated using SVM on a two-class problem. The first set (the "good" one) provides acceptable results, while the second one is far behind. When I merge the two sets...

12 March 2014 161 3 View

How do you specify CPSET and NSET for history output?

The simulation, I am trying to bring about, is essentially related to a contact analysis between deformable and analytical rigid bodies, the interaction between them is defined by means of surface...

13 February 2014 1,142 1 View

Dear colleagues! Do you think that all the team roles according to the Belbin model* will remain unchanged in the future?

Perhaps, some will cease to be relevant and, conversely, new ones will arise? And if the changes are still there, then what and why? (*Belbin model =...

01 January 1970 9,973 4 View

Transition from academic to business career: mission impossible?

Dear Colleagues! As far as, from your point of view, it is possible (and justified) to implement the concept of Life Long Learning in a professional academic career by completely withdrawing from...

01 January 1970 4,724 15 View

The need to disseminate information about the results of their scientific activities for the researcher in modern conditions.

Dear Colleagues! Do you think a modern researcher should independently make efforts to spread awareness in the world around him about the results of his scientific activity? If so, how much will...

01 January 1970 9,054 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View